Cuproptosis depends on intracellular copper accumulation and reveals nice potential in tumor remedy. Nevertheless, the excessive content material of glutathione (GSH) in tumor cells limits its effectiveness. Moreover, the mechanism of immune activation mediated by cuproptosis stays unclear. To handle this, we developed a most cancers cell membrane coated Cu₂O nanoparticle (TC) to induce cuproptosis in tumor cells. After getting into tumor cells by way of homologous concentrating on, the TC launched Cu²⁺ within the acidic microenvironment. Cu²⁺ are subsequently decreased to Cu+ and generate hydroxyl radicals via the Fenton response. The outcome led to the downregulation of GSH and finally sensitized cuproptosis. Microwave-induced hyperthermia additional amplifies these results. Experimental outcomes reveal that TC successfully induces 4T1 most cancers cells cuproptosis each in vitro and in vivo, considerably inhibiting 4T1 tumor development with minimal systemic toxicity. The remedy of TC additionally triggered tumor immunogenic cell dying and sensitized T-cell-mediated anti-tumor immunity. TC affords a promising technique for efficient most cancers cuproptosis and immunotherapy.
