Mierke, C. T. Extracellular matrix cues regulate mechanosensing and mechanotransduction of most cancers cells. Cells 13, 96 (2024).
Orr, A. W., Helmke, B. P., Blackman, B. R. & Schwartz, M. A. Mechanisms of mechanotransduction. Dev. Cell 10, 11–20 (2006).
Hoffman, B. D., Grashoff, C. & Schwartz, M. A. Dynamic molecular processes mediate mobile mechanotransduction. Nature 475, 316–323 (2011).
Jin, P., Jan, L. Y. & Jan, Y. N. Mechanosensitive ion channels: structural options related to mechanotransduction mechanisms. Annu Rev. Neurosci. 43, 207–229 (2020).
Romani, P., Valcarcel-Jimenez, L., Frezza, C. & Dupont, S. Crosstalk between mechanotransduction and metabolism. Nat. Rev. Mol. Cell Biol. 22, 22–38 (2021).
Grashoff, C. et al. Measuring mechanical rigidity throughout vinculin reveals regulation of focal adhesion dynamics. Nature 466, 263–266 (2010).
Hu, Y. et al. DNA-based ForceChrono probes for deciphering single-molecule pressure dynamics in residing cells. Cell 187, 3445–3459.e15 (2024).
Ren, Y. et al. Power redistribution in clathrin-mediated endocytosis revealed by coiled-coil pressure sensors. Sci. Adv. 9, eadi1535 (2023).
Tao, A. et al. Figuring out constitutive and context-specific molecular-tension-sensitive protein recruitment inside focal adhesions. Dev. Cell 58, 522–534.e7 (2023).
Zhang, Y., Ge, C., Zhu, C. & Salaita, Ok. DNA-based digital rigidity probes reveal integrin forces throughout early cell adhesion. Nat. Commun. 5, 5167 (2014).
Fisher, T. E., Oberhauser, A. F., Carrion-Vazquez, M., Marszalek, P. E. & Fernandez, J. M. The examine of protein mechanics with the atomic pressure microscope. Traits Biochem. Sci. 24, 379–384 (1999).
Bustamante, C. J., Chemla, Y. R., Liu, S. & Wang, M. D. Optical tweezers in single-molecule biophysics. Nat. Rev. Strategies Primers 1, 25 (2021).
Choi, H. Ok., Kim, H. G., Shon, M. J. & Yoon, T. Y. Excessive-resolution single-molecule magnetic tweezers. Annu. Rev. Biochem. 91, 33–59 (2022).
Ishijima, A. et al. Simultaneous commentary of particular person ATPase and mechanical occasions by a single myosin molecule throughout interplay with actin. Cell 92, 161–171 (1998).
del Rio, A. et al. Stretching single talin rod molecules prompts vinculin binding. Science 323, 638–641 (2009).
Dey, S. et al. DNA origami. Nat. Rev. Strategies Primers 1, 13 (2021).
Seeman, N. C. & Sleiman, H. F. DNA nanotechnology. Nat. Rev. Mater. 3, 17068 (2018).
Fisher, P. D. E. et al. A programmable DNA origami platform for organizing intrinsically disordered nucleoporins inside nanopore confinement. ACS Nano 12, 1508–1518 (2018).
Fu, J. et al. Multi-enzyme complexes on DNA scaffolds able to substrate channelling with a synthetic swinging arm. Nat. Nanotechnol. 9, 531–536 (2014).
Zeng, Y. C. et al. Advantageous tuning of CpG spatial distribution with DNA origami for improved most cancers vaccination. Nat. Nanotechnol. 19, 1055–1065 (2024).
Mills, A. et al. A modular spring-loaded actuator for mechanical activation of membrane proteins. Nat. Commun. 13, 3182 (2022).
Nickels, P. C. et al. Molecular pressure spectroscopy with a DNA origami-based nanoscopic pressure clamp. Science 354, 305–307 (2016).
Wang, Y. et al. A nanoscale DNA pressure spectrometer able to making use of rigidity and compression on biomolecules. Nucleic Acids Res. 49, 8987–8999 (2021).
Darcy, M. et al. Excessive-force utility by a nanoscale DNA pressure spectrometer. ACS Nano 16, 5682–5695 (2022).
Praetorius, F. et al. Biotechnological mass manufacturing of DNA origami. Nature 552, 84–87 (2017).
Jia, Y. L., Chen, L. M., Liu, J., Li, W. & Gu, H. Z. DNA-catalyzed environment friendly manufacturing of single-stranded DNA nanostructures. Chem 7, 959–981 (2021).
Kramm, Ok. et al. DNA origami-based single-molecule pressure spectroscopy elucidates RNA polymerase III pre-initiation complicated stability. Nat. Commun. 11, 2828 (2020).
Solar, Z., Guo, S. S. & Fassler, R. Integrin-mediated mechanotransduction. J. Cell Biol. 215, 445–456 (2016).
Goult, B. T., Yan, J. & Schwartz, M. A. Talin as a mechanosensitive signaling hub. J. Cell Biol. 217, 3776–3784 (2018).
Hytonen, V. P. & Vogel, V. How pressure may activate talin’s vinculin binding websites: SMD reveals a structural mechanism. PLoS Comput. Biol. 4, e24 (2008).
Yao, M. et al. Mechanical activation of vinculin binding to talin locks talin in an unfolded conformation. Sci. Rep. 4, 4610 (2014).
Yao, M. et al. The mechanical response of talin. Nat. Commun. 7, 11966 (2016).
Papagrigoriou, E. et al. Activation of a vinculin-binding web site within the talin rod includes rearrangement of a five-helix bundle. EMBO J. 23, 2942–2951 (2004).
Douglas, S. M. et al. Fast prototyping of 3D DNA-origami shapes with caDNAno. Nucleic Acids Res. 37, 5001–5006 (2009).
Funke, J. J. & Dietz, H. Inserting molecules with Bohr radius decision utilizing DNA origami. Nat. Nanotechnol. 11, 47–52 (2016).
Xiong, Q. et al. DNA origami post-processing by CRISPR-Cas12a. Angew. Chem. Int. Ed. 59, 3956–3960 (2020).
Aksel, T., Yu, Z., Cheng, Y. & Douglas, S. M. Molecular goniometers for single-particle cryo-electron microscopy of DNA-binding proteins. Nat. Biotechnol. 39, 378–386 (2021).
Douglas, S. M. et al. Self-assembly of DNA into nanoscale three-dimensional shapes. Nature 459, 414–418 (2009).
Wagenbauer, Ok. F. et al. How we make DNA origami. ChemBioChem 18, 1873–1885 (2017).
Poppleton, E. et al. Design, optimization and evaluation of huge DNA and RNA nanostructures by interactive visualization, modifying and molecular simulation. Nucleic Acids Res. 48, e72 (2020).
Woodside, M. T. et al. Nanomechanical measurements of the sequence-dependent folding landscapes of single nucleic acid hairpins. Proc. Natl Acad. Sci. USA 103, 6190–6195 (2006).
Liu, J. & Yan, J. Unraveling the dual-stretch-mode impression on rigidity gauge tethers’ mechanical stability. J. Am. Chem. Soc. 146, 7266–7273 (2024).
Bercy, M. & Bockelmann, U. Hairpins below rigidity: RNA versus DNA. Nucleic Acids Res. 43, 9928–9936 (2015).
Marko, J. F. & Siggia, E. D. Stretching DNA. Macromolecules 28, 8759–8770 (1995).
Kumar, A. et al. Talin rigidity sensor reveals novel options of focal adhesion pressure transmission and mechanosensitivity. J. Cell Biol. 213, 371–383 (2016).
Austen, Ok. et al. Extracellular rigidity sensing by talin isoform-specific mechanical linkages. Nat. Cell Biol. 17, 1597–1606 (2015).
Chung, M., Zhou, Ok., Powell, J. T., Lin, C. & Schwartz, M. A. DNA-based molecular clamp for probing protein interactions and construction below pressure. ACS Nano 18, 27590–27596 (2024).
Lin, C., Perrault, S. D., Kwak, M., Graf, F. & Shih, W. M. Purification of DNA-origami nanostructures by rate-zonal centrifugation. Nucleic Acids Res. 41, e40 (2013).
Abramson, J. et al. Correct construction prediction of biomolecular interactions with AlphaFold 3. Nature 630, 493–500 (2024).
Carrion-Vazquez, M. et al. Mechanical and chemical unfolding of a single protein: a comparability. Proc. Natl Acad. Sci. USA 96, 3694–3699 (1999).
Evans, E. & Ritchie, Ok. Energy of a weak bond connecting versatile polymer chains. Biophys. J. 76, 2439–2447 (1999).
Zhou, J., Kang, X., An, H., Lv, Y. & Liu, X. The perform and pathogenic mechanism of filamin A. Gene 784, 145575 (2021).
Kumar, A. et al. Filamin A mediates isotropic distribution of utilized pressure throughout the actin community. J. Cell Biol. 218, 2481–2491 (2019).
Aissaoui, N. et al. Modular imaging scaffold for single-particle electron microscopy. ACS Nano 15, 4186–4196 (2021).
Pfaff, M., Liu, S., Erle, D. J. & Ginsberg, M. H. Integrin beta cytoplasmic domains differentially bind to cytoskeletal proteins. J. Biol. Chem. 273, 6104–6109 (1998).
Rief, M., Clausen-Schaumann, H. & Gaub, H. E. Sequence-dependent mechanics of single DNA molecules. Nat. Struct. Biol. 6, 346–349 (1999).
Amiram, M. et al. Evolution of translation equipment in recoded micro organism permits multi-site incorporation of nonstandard amino acids. Nat. Biotechnol. 33, 1272–1279 (2015).
Zadeh, J. N. et al. NUPACK: evaluation and design of nucleic acid techniques. J. Comput. Chem. 32, 170–173 (2010).
Driscoll, T. P., Ahn, S. J., Huang, B., Kumar, A. & Schwartz, M. A. Actin flow-dependent and -independent pressure transmission by integrins. Proc. Natl Acad. Sci. USA 117, 32413–32422 (2020).
Chanduri, M. et al. Mobile stiffness sensing by talin 1 in tissue mechanical homeostasis. Sci. Adv. 10, eadi6286 (2024).
Bepler, T. et al. Optimistic-unlabeled convolutional neural networks for particle choosing in cryo-electron micrographs. Nat. Strategies 16, 1153–1160 (2019).
Yan, J., Yao, M., Goult, B. T. & Sheetz, M. P. Talin dependent mechanosensitivity of cell focal adhesions. Cell. Mol. Bioeng. 8, 151–159 (2015).